effects of gnrha active immunity on fshr expression and uterine development in prepuberty and non cyclic ewes
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abstract
this study explored the effects of gnrh agonist (gnrha) immunity on expression of fshr mrna in pituitary and fshr protein in uteri, and also confirmed its efficacy on uterine development. 42 prepuberty and non cyclic ewes were assigned to 6 experimental groups (eg) during anestrus season. animals in eg-i, eg-ii and eg-iii were subcutaneously injected with 200, 300 and 400 μg alarelin antigens (day 0 and 14). animals in eg-iv and eg-v were subcutaneously injected with 200 and 300 μg alarelin antigen (day 0, 7, 14 and 21). uterine fshr protein expression was detected using western-blotting. rt-pcr was implemented tomeasure expression of pituitary fshr mrna on day 70 after the immunity. compared to the cg, pituitary fshr mrna levels reduced by 58%, 88% and 91% (p
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Journal title:
iranian journal of veterinary researchPublisher: shiraz university
ISSN 1728-1997
volume 15
issue 3 2014
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